Fig. 1: CREB3-FL is directly localized to the nuclear membrane and not to the canonical ER/Gc transport or S1P/S2P cleavage pathway.
From: Dysregulated CREB3 cleavage at the nuclear membrane induces karyoptosis-mediated cell death
a Illustration showing the localization of CREB3-FL and CREB3-CF and changes in nuclear morphology. b Western blotting showing the localization of endogenous CREB3-FL and CREB3-CF in the cytosolic and nuclear fractions. c Illustration showing the nuclear membrane localization of CREB3-FL and CREB3-FL-mtS1P. d Left panels: Diagram showing the experimental strategies used to obtain the extracts of the cytosolic and nuclear fractions. Right panels, Western blotting showing nuclear DNA bound to CREB3-FL, CREB3-CF, and CREB3-dTM. e Confocal microscopy image illustrating the nuclear membrane localization of the Myc-CREB3-FL-HA double-tagged protein. f Diagram showing the experimental strategy used to determine the extent of nuclear DNA binding to CREB3-FL, CREB3-CF, and CREB3-FL-mtS1P. g Western blotting showing nuclear DNA binding of CREB3-FL, CREB3-CF, and CREB3-FL-mtS1P in the presence of NaCl. h Illustration of the localization and orientation of CREB3 at the nuclear membrane.
