Fig. 1: High expression of CERK is involved in CCl4-induced acute liver injury. | Experimental & Molecular Medicine

Fig. 1: High expression of CERK is involved in CCl4-induced acute liver injury.

From: Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2

Fig. 1

a Representative images of liver injury sites stained with hematoxylin and eosin (H&E) are shown. Scale bar: 250 μm. b qRT‒PCR analysis of the seven top genes involved in sphingolipid metabolism (fold change>6) in the validation groups was performed. (n = 5/group). c qRT‒PCR analysis of CERK expression was performed in acute liver injury time course experiments. (n = 5/group). d The protein expression of CERK in acute liver injury time course experiments was determined by western blotting. e Representative images of IHC staining for CERK in tissues at different time points after acute liver injury are shown. The arrow indicates severely injured hepatocytes, and the asterisk indicates mildly injured hepatocytes. Scale bar: 500 μm (top), 100 μm (bottom). One-way ANOVA was used to analyze the significant differences. **P< 0.01, ***P< 0.001, and ****P < 0.0001.

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