Fig. 3: CERK knockdown exacerbated acute liver injury in vitro. | Experimental & Molecular Medicine

Fig. 3: CERK knockdown exacerbated acute liver injury in vitro.

From: Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2

Fig. 3

a The mRNA expression of CERK in AML12 cells with stable CERK knockdown was measured by qRT‒PCR. b The protein expression of CERK in AML12 cells with stable CERK knockdown was measured by western blotting. c The relative concentrations of total C1P in AML12 cells with stable CERK knockdown were measured via ELISA. d The viability of stable CERK-knockdown AML12 cells treated with C1P. e The level of intracellular ROS in AML12 cells with stable CERK knockdown was measured via an ROS assay kit. a, c Student’s t test; d one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, and ns not significant.

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