Fig. 5: CERK and C1P do not lead to changes in KEAP1 expression, which is not involved in the upregulation of NRF2 protein expression induced by CERK/C1P. | Experimental & Molecular Medicine

Fig. 5: CERK and C1P do not lead to changes in KEAP1 expression, which is not involved in the upregulation of NRF2 protein expression induced by CERK/C1P.

From: Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2

Fig. 5

a The protein expression of KEAP1 in mice with acute liver injury was measured via WB at different time points. b The mRNA expression of KEAP1 in mice with acute liver injury was measured by qRT‒PCR at different time points. c The protein expression of KEAP1 in AML12 cells with oxidative damage induced by different concentrations of H2O2 was measured via western blotting. d The mRNA expression of KEAP1 in AML12 cells with oxidative damage induced by different concentrations of H2O2 was measured via qRT‒PCR. e The protein expression of KEAP1 in AML12 cells with stable CERK knockdown was measured by western blotting. f The mRNA expression of KEAP1 in AML12 cells with stable CERK knockdown was measured by qRT‒PCR. g The protein expression of KEAP1 in AML12 cells treated with NVP-231 (upper panel) or C1P (lower panel) was measured via western blotting. h The mRNA expression of KEAP1 in AML12 cells treated with NVP-231 or C1P was measured by qRT‒PCR. One-way ANOVA was used to analyze the significant differences. ns not significant.

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