Fig. 6: CERK and C1P facilitate the activation of NRF2 by promoting its dissociation from KEAP1.

a The protein expression of NRF2 and KEAP1 in the cytoplasm and nucleus of AML12 cells treated with C1P or NVP-231 was measured via WB. The negative control groups were not exposed to H₂O₂, while the vehicle, C1P, and NVP-231 groups were exposed to H₂O₂. b The interaction between KEAP1 and NRF2 in AML12 cells treated with C1P or NVP-231 was assessed by co-IP. The negative control groups (lane 2) were incubated with the same quality rabbit IgG as the co-IP baits. c Fluorescence images of the colocalization of KEAP1 and NRF2 in AML12 cells treated with C1P or NVP-231 were obtained via IF. d The protein expression of NRF2 and KEAP1 in the cytoplasm and nucleus of AML12 cells with stable CERK knockdown was measured via western blotting. e The interaction between KEAP1 and NRF2 in stable CERK-knockdown AML12 cells was assessed by co-IP. The negative control groups (lane 2) were incubated with the same quality rabbit IgG as the co-IP baits. f IF images of the colocalization of KEAP1 and NRF2 in AML12 cells with stable CERK knockdown were obtained. g The protein expression of NRF2 in AML12 cells cotreated with 2 μM NVP-231 and 4-OI was measured by western blotting. h The protein expression of NRF2 in stable CERK-knockdown AML12 cells treated with 4-OI was measured by western blotting. One-way ANOVA was used to analyze the significant differences. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, not significant.