Fig. 2: Glutamine-derived aspartate promotes TAM polarization. | Experimental & Molecular Medicine

Fig. 2: Glutamine-derived aspartate promotes TAM polarization.

From: Glutamine-derived aspartate is required for eIF5A hypusination-mediated translation of HIF-1α to induce the polarization of tumor-associated macrophages

Fig. 2: Glutamine-derived aspartate promotes TAM polarization.

a Relative glutamine level in TAMs and M2 macrophages. b Levels of SNAT1, SNAT2, and ASCT2 in TAMs and M2 macrophages. c, d Protein levels (c) and relative expression levels of the mRNAs (d) encoding HIF-1α, ARG1, CD80, and CD204 in TAMs and low-glutamine-conditioned TAMs. e, f HIF-1α, ARG1, CD80, and CD204 mRNA (e) and protein (f) expression levels in TAMs in the presence or absence of BPTES. g Relative aspartate level in TAMs in the presence or absence of BPTES. h, i Protein levels (h) and relative expression levels of the mRNAs encoding (i) HIF-1α, ARG1, CD80, and CD204 in BPTES-treated TAMs in the presence or absence of aspartate. The data are expressed as the mean ± SEM of three independent experiments. N.S. not significant; *p < 0.05; **p < 0.01; and ***p < 0.001. CM conditioned medium, Gln glutamine, Asp aspartate.

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