Fig. 4: Psoriatic skin inflammation induces the degranulation of small intestinal eosinophils.
a Quantitative PCR analysis. SI, small intestine; LI, large intestine. b Immunohistochemical staining for eosinophil peroxidase (EPX) (upper row) in the SI and EPX levels in the serum and stool (lower row). Scale bars, 134 μm. c Representative flow cytometry plots (left) and percentages (right) of CCR3+CD63+ and CCR3+SIRPα+ eosinophils in the SI. Singlet SSChighCD45+CD11b+ cells were gated. d Photographs of skin treated with vehicle cream (control) or imiquimod (IMQ). e Hematoxylin and eosin-stained skin. Scale bars, 124.5 μm. f Epidermal thickness. Three distinct regions of each section were analyzed. g Quantitative PCR analysis of the skin. h EPX immunohistochemical staining and quantification in the SI. The intensity of each section was analyzed. Scale bars, 124.5 μm. i Quantitative PCR analysis of the SI. j Schematic of Caco-2 cell treatment with conditioned media (CM) collected from AML 14.3D10 cells. k Viability of Caco-2 cells. l Quantitative PCR analysis of Caco-2 cells. m Transepithelial electrical resistance (TEER) of polarized Caco-2 cells. ****P < 0.0001 vs. control media. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 according to the unpaired t test (a, b, f, g, h, and i), Mann–Whitney test (c and Il1b in i), one-way ANOVA with Tukey’s multiple comparisons (k and l), or two-way ANOVA with Bonferroni’s multiple comparisons (m).
