Fig. 1: Vhl cKO partially protects mice from STZ-induced T1DM.
a Random and fasting blood glucose levels of the 8-week-old control and STZ-treated mice (n = 5). b Random and fasting blood glucose levels of the 8-week-old Vhl cKO mice (n = 5) and their littermate controls (n = 5) after STZ injection. c GTTs of the 8-week-old Vhl cKO mice (n = 5) and their littermate controls (n = 5) after STZ injection. d Serum insulin levels of the 8-week-old Vhlflox/flox mice (n = 5), Vhl cKO mice (n = 5), STZ-treated Vhlflox/flox mice (n = 8) and STZ-treated Vhl cKO mice (n = 7). e The Vhl cKO mice exhibited greater glucose-stimulated insulin secretion (GSIS) than the control mice under STZ conditions. f HE staining and insulin immunostaining of pancreatic islets from the 8-week-old Vhl cKO mice and their littermate controls after STZ injection. g mRNA expression of insulin in the pancreatic islets of the 8-week-old Vhl cKO mice and their littermate controls after injection with STZ. h, j BAX IHC of pancreatic islets from the 8-week-old Vhl cKO mice and their littermate controls after STZ injection. i, k PCNA IHC of pancreatic islets from the 8-week-old Vhl cKO mice and their littermate controls after STZ injection (n = 5). l The islet equivalent (IEQ) of the Vhl cKO mice was greater than that of their control littermates after STZ treatment (n = 5). m Body weight changes in the Vhl cKO mice and their littermate controls after STZ injection. All data are presented as the mean ± SEM, and p values were analyzed by two-tailed t-tests in (a, j–l); one-way ANOVA in (b, d, g); and two-way ANOVA in (c, e, m). *p < 0.05, ***p < 0.001. All the data are representative of two to three independent experiments. The data were obtained from male mice.
