Fig. 8: MT3 modulates osteoclast differentiation through SP1-mediated regulation of Nfatc1. | Experimental & Molecular Medicine

Fig. 8: MT3 modulates osteoclast differentiation through SP1-mediated regulation of Nfatc1.

From: Unique expression and critical role of metallothionein 3 in the control of osteoclastogenesis and osteoporosis

Fig. 8

a Genome track view of osteoclast ATAC-seq profiles (GSE211671) for Nfatc1. The region on Chr18:80712933-80713289 is highlighted with a pink background. b A representative image of ChIP-DNA electrophoresis. c ChIP‒qPCR results showing SP1 binding to the Nfatc1 promoter (n = 3). d Analysis of the Nfatc1 promoter region from (a) using JASPAR to identify potential SP1-binding sites. RS, relative score. e Schematic of DN-SP1. f Expression levels of Sp1 and Nfatc1 mRNA in BMMs infected with retroviruses carrying pMX-IG-DN-SP1 or pMX-IG followed by culture with RANKL (n = 3). g Representative stained images and quantification of TRAP-positive multinucleated cells (n = 4). h A schematic model depicting the role of MT3 in controlling excessive osteoclastogenesis.

Back to article page