Fig. 3: CMTM6 knockdown reduces CRC tumor growth in nude mice and the Glut1 protein level in CRC cells.
From: CMTM6 mediates the Warburg effect and promotes the liver metastasis of colorectal cancer
a Control and CMTM6-knockdown HCT116 cells were mixed with liver fibroblasts, and they were subcutaneously coinjected into immunosuppressed nude mice. CMTM6 knockdown suppressed HCT116 tumor growth in nude mice. ****, P < 0.0001 according to ANOVA, n = 7,8. b Cryo-sections of HCT116 tumors were subjected to immunofluorescence (IF) staining for Ki67 (left) or cleaved caspase 3 (right). The cell nuclei were counterstained with DAPI (blue). The percentage of Ki67-positive cells was lower whereas the percentage of cleaved-caspase 3-positive cells was higher in CMTM6-knockdown tumors than in control tumors. ****, P < 0.0001 by t test, n = 7, 8. Scale bar, 50 μm. c, d IF and WB revealed reduced protein levels of glucose transporter 1 (Glut1) in CMTM6-knockdown tumors compared to control tumors. **, P < 0.01; ****, P < 0.0001 by t test, n = 4,4. Scale bar, 50 μm. e WB showed that Glut1 protein levels in CRC cells were reduced by CMTM6 knockdown in 2D culture. **, P < 0.01 according to ANOVA, n = 3.
