Fig. 4: CMTM6 is required for the plasma membrane (PM) localization of Glut1, glucose uptake, and glycolysis in CRC cells.
From: CMTM6 mediates the Warburg effect and promotes the liver metastasis of colorectal cancer
a A glucose uptake assay followed by flow cytometry analysis of 2-NBDG fluorescence demonstrated that the percentage of HCT116 cells that were positive for 2-NBDG uptake was reduced by CMTM6 knockdown. b The average 2-NBDG uptake by HCT116 cells was reduced by CMTM6 knockdown. **, P < 0.001 according to ANOVA; n > 5000 cells per group. c Glucose uptake assays showed that the percentage of MC38 cells that were positive for 2-NBDG uptake was reduced by CMTM6 knockdown. d The average 2-NBDG uptake by MC38 cells was reduced by CMTM6 knockdown. **, P < 0.01 by t test; n > 5000 cells per group. e The Agilent Seahorse Glycolysis Stress test was performed, and changes in the extracellular acidification rate (ECAR) were recorded in real time. Glycolysis in HCT116 cells was reduced by CMTM6 knockdown. ****, P < 0.0001 according to ANOVA, n = 5. f The Seahorse glycolysis stress test showed that glycolysis in MC38 cells was suppressed by CMTM6 knockdown. *, P < 0.05 according to ANOVA, n = 5. g A biotinylation assay revealed that the Glut1 level in the PM of HCT116 cells was reduced by CMTM6 knockdown. ***, P < 0.001 by t test, n = 3.
