Fig. 1: High-resolution holotomograms of live mouse sIOs exhibiting detailed structures.

a Overview of the low-coherence HT workflow. b, c Representative holotomograms of sIOs displaying two distinct morphologies: enterocystic organoids (b) and multilobular organoids (c). The 3D rendered images are optically sectioned within a cubic box, with each axial slice on the right-hand side delineated by colored dashed boxes. Scale boxes represent a volume of 10 µm × 10 µm × 10 µm (width, height, and depth, respectively). d Detailed subcellular-level structures. The red dotted lines represent cell boundaries. e Macroscopic structures revealed in stitched sIO holotomograms. The black arrowheads represent the brush borders of the sIOs. The orange arrows indicate exfoliated cells and debris from dead cells. All the scale bars in this figure represent 20 µm.