Fig. 3: Quantitative evaluation of drug responsiveness in sIOs via low-coherence HTs. | Experimental & Molecular Medicine

Fig. 3: Quantitative evaluation of drug responsiveness in sIOs via low-coherence HTs.

From: Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids via low-coherence holotomography

Fig. 3

a Diagram illustrating holotomogram-based viability assessment of drug-treated sIOs. The red arrowheads highlight the time points at which the images were captured. b Time-lapse images of sIOs following drug treatment. Each colored box is magnified, with the color bar indicating sample depth. Scale bar = 20 µm. c–e Longitudinal quantitative assessment of volume (c), protein density (d), and protein mass (e). Every parameter is calibrated against the initial measurement; the dotted line signifies the baseline value.

Back to article page