Fig. 4: In-depth quantitative analysis of sIO viability.

a Illustration of the subvolume sectioning strategy accompanied by representative images. Scale bar = 20 µm. b Scatter plot of the protein density of vehicle- and cisplatin-treated sIOs. Each dot represents a subvolume of sectioned images (N = 1053 for vehicle, N = 1005 for cisplatin). The bottom and top of each box signify the 25th and 75th percentiles of the subvolumes, respectively. The distance between the bottom and top of each box gives the interquartile range, and the bold line in the middle of each box indicates the median. The t test results are as indicated; ***p < 0.001. c Correlative images of sIOs labeled with Hoechst (blue) and calcein-AM (green). In the merged images, the magenta and orange boxes indicate live cell-dominant and dead cell-dominant regions, respectively. Scale bar = 50 µm. d Histograms illustrating the protein density distribution within the boxed regions of (c). Each color corresponds to the color of the box.