Fig. 1: The SUMOylation of BRD4 is elevated in the synovium of patients diagnosed with PTOA. | Experimental & Molecular Medicine

Fig. 1: The SUMOylation of BRD4 is elevated in the synovium of patients diagnosed with PTOA.

From: Cbx4 SUMOylates BRD4 to regulate the expression of inflammatory cytokines in post-traumatic osteoarthritis

Fig. 1

a Representative images of Safranin O-fast green and Alcian blue staining of cartilage and synovial tissues from 2 patients. PTF (R): comminuted fracture of the tibial plateau (right); KOA (L): knee osteoarthritis (left); b The protein levels of IL-1β, TNF-α, IL-6, BRD4, and Cbx4 in patient articular cartilage and synovial tissues determined via IHC. c The level of SUMO-modified BRD4 determined via co-IP in the immunoprecipitates of SUMO1 and SUMO2/3 from patient articular synovial tissues. d The levels of SUMO1 and SUMO2/3 in BRD4 immunoprecipitates from patients’ articular synovial tissues were determined via co-IP, which revealed the presence of SUMO-modified BRD4 products. e The endogenous binding relationship between BRD4 and Cbx4 in patient synovial tissues was determined via co-IP. In c–e, mixed samples within the group, n = 5.

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