Fig. 5: Overexpressing FTO or SMAD2 partially alleviated OA progression in the DMM mouse model.
From: FTO-mediated SMAD2 m6A modification protects cartilage against Osteoarthritis
a mRNA expression levels of COL2A1, Aggrecan, SOX9, MMP3 and MMP13 in primary chondrocytes isolated from FTO-cKO mice cultured and transfected with Cre adenovirus (or vector) and FTO (or vector). b Western blot detection of FTO, SMAD2, COL2A1, Aggrecan, SOX9, MMP3 and MMP13 in primary chondrocytes isolated from FTO-cKO mice cultured and transfected with Cre adenovirus (or vector) and FTO (or vector). c Safranin O/Fast Green staining and immunohistochemistry (FTO, SMAD2, COL2A1 and MMP13) of the knee joints of FTOfl/fl, Col2a1-CreERT2 mice that underwent DMM. Articular injection of FTO adeno-associated virus (AAV) or vector. n = 8 per group. Scale bars, 100 μm and 20 μm. d Micro-CT images of the knee joints of FTO-cKO mice that underwent DMM surgery. Articular injection of FTO adeno-associated virus (AAV) or vector. Yellow arrows indicate the osteophytes. e The hot plate and rotarod test results of FTO-cKO mice that underwent DMM surgery. Articular injection of FTO adeno-associated virus (AAV) or vector. f mRNA expression levels of COL2A1, Aggrecan, SOX9, MMP3 and MMP13 in primary chondrocytes isolated from FTO-cKO mice cultured and transfected with Cre adenovirus (or vector) and SMAD2 (or vector). g Western blot detection of FTO, SMAD2, COL2A1, Aggrecan, SOX9, MMP3 and MMP13 in primary chondrocytes isolated from FTO-cKO mice cultured and transfected with Cre adenovirus (or vector) and SMAD2 (or vector). h Safranin O/Fast Green staining and immunohistochemistry (FTO, SMAD2, COL2A1 and MMP13) of the knee joints of FTOfl/fl, Col2a1-CreERT2 mice that underwent DMM. Articular injection of SMAD2 adeno-associated virus (AAV) or vector. n = 8 per group. Scale bars, 100 μm and 20 μm. i Micro-CT images of the knee joints of FTO-cKO mice that underwent DMM surgery. Articular injection of SMAD2 adeno-associated virus (AAV) or vector. Yellow arrows indicate the osteophytes. j The hot plate and rotarod test results of FTO-cKO mice that underwent DMM surgery. Articular injection of SMAD2 adeno-associated virus (AAV) or vector. The data are representative of three independent experiments (b, g). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, mean ± SD, two-tailed t test.
