Fig. 5: CLIP1 rescues the damaging effects of TFPI2. | Experimental & Molecular Medicine

Fig. 5: CLIP1 rescues the damaging effects of TFPI2.

From: Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver

Fig. 5

a‒d Western blotting and analysis of TFPI2, CLIP1, IL-1β, TNF-α, HMGB1, p65, p-p65, lkB, p-lkB, and β-actin proteins in HUVECs treated with LPS and transfected with TFPI2, CLIP1, or empty vector. e–g The concentrations of IL-1β, IL-6, and TNF-α in the culture medium of HUVECs treated with LPS and transfected with TFPI2, CLIP1, or empty vector were determined via ELISA. h NF-κB-responsive reporter activity in HEK293T cells transfected with TFPI2 and CLIP1. i and j Apoptosis of HUVECs treated with LPS and transfected with TFPI2, CLIP1, or empty vector detected by flow cytometry. n = 3 per group; the data are presented as the mean ± SD; ns not significant; *P < 0.05; and **P < 0.01.

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