Fig. 1: Different antiproliferative effects of stearate and palmitate on various cancer cell lines. | Experimental & Molecular Medicine

Fig. 1: Different antiproliferative effects of stearate and palmitate on various cancer cell lines.

From: A stearate-rich diet and oleate restriction directly inhibit tumor growth via the unfolded protein response

Fig. 1

a Clustering analysis of the results of the proliferation assay. Data from MTT proliferation assays of samples, including lung, breast, ovarian, and colon cancer cell lines, were subjected to k-means clustering analysis based on absorbance. The cells were cultured for 72 h and treated with 50 µM stearate or palmitate, followed by measurement using an MTT assay. The results were normalized to those of the fatty acid-free control. The ovarian cancer cell lines are highlighted in red (n = 6). b MTT assay of ovarian cancer cell lines. Ovarian cancer cell lines (OVCAR5, OVCAR8, SKOV3, ES-2, and OVCAR3) were treated with 50 µM free fatty acids (palmitate, palmitoleate, stearate, or oleate) for 72 h and then subjected to an MTT assay. Proliferation was assessed every 24 h. Data are presented as the means ± SEMs (n = 4; *p < 0.05, Mann–Whitney test). c Dose‒response curves for various fatty acids in different ovarian cancer cell lines. Representative dose‒response curves for stearate, palmitate, and oleate are shown based on quadruplicate data.

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