Fig. 3: The inhibition of unsaturation increases stearate toxicity, and exogenous oleate mitigates it. | Experimental & Molecular Medicine

Fig. 3: The inhibition of unsaturation increases stearate toxicity, and exogenous oleate mitigates it.

From: A stearate-rich diet and oleate restriction directly inhibit tumor growth via the unfolded protein response

Fig. 3

a Proposed functional mechanism of SCD in stearate metabolism. shRNA-mediated knockdown and CAY10566 were used to inhibit SCD, the enzyme that converts stearate to oleate. b IC50 values of stearate in shSCD-treated OVCAR5 cells. SCD knockdown lowered the IC50 (n = 6; *p < 0.05 and **p < 0.01, Mann–Whitney test). c Analogous IC50 findings in shSCD-treated OVCAR8 cells (n = 6; *p < 0.05 and **p < 0.01, Mann–Whitney test). d Effects of oleate on the viability of stearate-treated shSCD-transfected OVCAR5 cells. The cells were treated with the indicated concentrations of stearate and coincubated with 50 µM oleate for 72 h; viability was measured via an MTT assay (n = 6; *p < 0.05 and **p < 0.01, Mann–Whitney test). IC50 values of stearate in OVCAR5 (e) and OVCAR8 (f) cells treated with 1 μM CAY10566 or the DMSO control (n = 6; **p < 0.01, Mann–Whitney test). g Cell viability in response to treatment with stearate and oleate in the presence of 1 μM CAY10566. The cells were treated with the indicated concentrations of stearate and coincubated with 50 µM oleate for 72 h; viability was assessed after 72 h via an MTT assay (n = 6; *p < 0.05, Mann–Whitney test).

Back to article page