Fig. 4: Stearate induces cytotoxicity via ER stress and CHOP activation. | Experimental & Molecular Medicine

Fig. 4: Stearate induces cytotoxicity via ER stress and CHOP activation.

From: A stearate-rich diet and oleate restriction directly inhibit tumor growth via the unfolded protein response

Fig. 4

a RNA-Seq of OVCAR5 cells subjected to various treatments. The cells were treated and cultured for 24 h before RNA-seq. Principal component analysis revealed distinct gene expression profiles without treatment-based separation. b Top 10 functionally enriched terms. The biological processes induced by stearate compared with those induced by DMSO are shown. Representative western blot analysis of proteins involved in the unfolded protein response (UPR), apoptosis, and DNA damage in OVCAR5 (c) and OVCAR8 (d) cells treated with the indicated concentrations of stearate and oleate. GAPDH was used as an internal control. Representative western blot analysis of protein expression following the knockdown of CHOP (shCHOP) in OVCAR5 (e) and OVCAR8 (f) cells. α-Tubulin was used as an internal control. IC50 values of stearate in shCHOP-transfected OVCAR5 (g) and OVCAR8 (h) cells (n = 6; *p < 0.05 and **p < 0.01, Mann–Whitney test). IC50 values of stearate in OVCAR5 (i) and OVCAR8 (j) cells treated with 5 μM 4-PBA or the DMSO control (n = 6; **p < 0.01, Mann–Whitney test).

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