Fig. 6: The inhibition of unsaturation along with dietary supplementation with stearate hinders tumor growth, which is reversed by the addition of oleate. | Experimental & Molecular Medicine

Fig. 6: The inhibition of unsaturation along with dietary supplementation with stearate hinders tumor growth, which is reversed by the addition of oleate.

From: A stearate-rich diet and oleate restriction directly inhibit tumor growth via the unfolded protein response

Fig. 6

Tumor growth in mice following subcutaneous injection of control (shCtr) (a) or SCD-knockdown (shSCD) (b) OVCAR5 cells. The mice were divided into three dietary groups, namely, the normal-fat diet (NFD), O-HFD, and S-HFD groups, and received the intervention from 3 days before injection until the end of the study (n = 6). The data are presented as the means ± SEMs. *p < 0.05, **p < 0.01, and ns stands for not significant; the Mann–Whitney test was applied between shCtr samples and the Wilcoxon matched-pairs signed rank test was applied between shCtr and shSCD-1 pairs. c Weights of the tumors at the end of the experimental period. d–f Validation of the results using OVCAR8 cells (n = 6). The data are presented as the means ± SEMs. *p < 0.05, **p < 0.01, and ns stands for not significant; the Mann–Whitney test was applied between shCtr samples and the Wilcoxon matched-pairs signed rank test was applied between shCtr and shSCD-1 pairs. g Representative images of immunohistochemical staining of tumor tissues derived from shCtr-OVCAR5 cells or shSCD-1-OVCAR5 cells depicting the levels of cleaved caspase-3, γH2AX, and CHOP. h–j Quantitative analysis of cleaved caspase-3, γH2AX, and CHOP levels in tissue. (n = 30; ***p < 0.001, ns: not significant, Mann–Whitney test).

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