Fig. 2: Exploration of pro-metastatic characteristics of epithelial cells in metastatic ESCC. | Experimental & Molecular Medicine

Fig. 2: Exploration of pro-metastatic characteristics of epithelial cells in metastatic ESCC.

From: Single-cell RNA sequencing and spatial transcriptomics of esophageal squamous cell carcinoma with lymph node metastases

Fig. 2

a UMAP plots of the 16,416 high-quality cells from all the 21 samples, with each color coded by either cluster of epithelial cells. b The proportion of each epithelial cell cluster in different samples. c CNV scores of epithelial cells obtained from tumor samples, metastatic lymph nodes and adjacent tissues. d Heatmap depicting key marker genes for each subcluster of epithelial cells. e Heatmap showing relative expression levels of metastasis-related genes identified in metastatic ESCC. f Boxplot showing the malignant and non-malignant scores of each subcluster. g Specific GO functions enriched in epithelial cells obtained from metastatic lymph nodes in ESCC. h Representative H&E staining of tumor samples biopsied for spatial transcriptomics and spatial distribution of Metastatic Score, EPCAM, ASPH and TXNRD1 in the tumor site of one patient with metastatic lymph nodes. i The mIHC staining of EPCAM, DSP and TXNRD1 in the tumor site of one patient with metastatic lymph nodes. Scale bars: 100 μm.

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