Fig. 3: Identification of cancer-associated fibroblasts correlated with collagen reduction and angiogenesis in metastatic ESCC. | Experimental & Molecular Medicine

Fig. 3: Identification of cancer-associated fibroblasts correlated with collagen reduction and angiogenesis in metastatic ESCC.

From: Single-cell RNA sequencing and spatial transcriptomics of esophageal squamous cell carcinoma with lymph node metastases

Fig. 3

a UMAP plots showing 14,248 high-quality cells from all the 29 samples, with each color coded by either main annotated cell type. b Bubble plot showing key marker genes for each subcluster of fibroblasts. c Heatmap comparing the proportion of fibroblasts among tumor samples, adjacent normal tissue, metastatic lymph nodes and non-metastatic lymph nodes. *P < 0.05; **P < 0.01; ***P < 0.001. d Violin plots showing the expression levels of markers correlated with collagen reduction and angiogenesis in different subpopulations. e Heatmap depicting the expression levels of angiogenesis correlated genes in different fibroblast subpopulations. f Bar plot showing the GO functions enriched in mCAF1 subpopulation. g Spatial distribution of mCAF1 subpopulation and MMP3, MMP1 and CXCL8 in the tumor site of one patient with metastatic lymph nodes. h The mIHC staining of MMP3, POSTN and IL-24 in the tumor site of one patient with metastatic lymph nodes. Scale bars: 100 μm.

Back to article page