Fig. 4: Astrocytic GABA-T overexpression is sufficient to increase GABA clearance and reduce tonic inhibition in dentate GCs. | Experimental & Molecular Medicine

Fig. 4: Astrocytic GABA-T overexpression is sufficient to increase GABA clearance and reduce tonic inhibition in dentate GCs.

From: Suppressing astrocytic GABA transaminase enhances tonic inhibition and weakens hippocampal spatial memory

Fig. 4: Astrocytic GABA-T overexpression is sufficient to increase GABA clearance and reduce tonic inhibition in dentate GCs.

a, Immunostaining for GABA-T and GFAP in the control and astrocytic GABA-T-overexpressing (OE) groups. The GFP signal indicates the expression of the virus. The magnified inset image shows the astrocyte indicated by the yellow arrowhead (inset scale bar, 20 μm). b, Violin plots comparing the average intensity of GABA-T staining in GFAP+ areas between the two groups. c, Representative traces of GABAAR-mediated phasic and tonic GABA currents from the control and astrocytic GABA-T OE groups. d, Comparative bar graphs of the tonic GABA current (left), sIPSC amplitude (middle) and sIPSC frequency (right). sIPSCs recorded before the bicuculline (Bic) treatment were analyzed. n = 3 mice per group. The data are presented as the mean ± standard error of the mean. The individual dots refer to cells, unless otherwise specified. The P values were obtained via unpaired t-tests for d (left and middle) and Mann‒Whitney tests for b and d (right). **P < 0.01, ****P < 0.0001; nonsignificant (ns) indicates a P > 0.05.

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