Fig. 5: Enhanced tonic inhibition due to GABA-T suppression is not driven primarily by synaptic GABA spillover in dentate GCs.
a, Representative traces of GABAAR-mediated phasic and tonic GABA currents from the control and general GABA-T KD groups treated with TTX and bicuculline (Bic). TTX-sensitive tonic GABA currents were measured by the baseline shift from the gray dotted line to the red dotted line. TTX-insensitive tonic GABA currents were measured by the baseline shift from the red dotted line to the blue dotted line. b, Bar graphs comparing TTX-sensitive and TTX-insensitive tonic GABA currents between the control and general GABA-T KD groups. n = 3 mice per group. c, Paired scatter plots showing the IPSC frequency (left) and IPSC amplitude (right) in the control and general GABA-T KD groups before and after TTX treatment. n = 3 mice per group. The data are presented as the mean ± standard error of the mean. The individual dots refer to cells, unless otherwise specified. The P values were obtained via two-way ANOVA (Šídák’s multiple comparisons test) for b and a two-way repeated-measures ANOVA (uncorrected Fisher’s LSD) for c. **P < 0.01, ****P < 0.0001; nonsignificant (ns) indicates a P > 0.05.
