Fig. 5: Osteoclast activation reveals the enhanced expression of migratory module, NBC. | Experimental & Molecular Medicine

Fig. 5: Osteoclast activation reveals the enhanced expression of migratory module, NBC.

From: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop

Fig. 5

a Relative mRNA levels of Slc4a4, Slc4a7 and Slc4a10 in Raw264.7 cells treated with or without M-CSF and RANKL for 24 h. The bars represent the mean ± s.e.m. (n = 4). b NBCn1 protein levels in Raw264.7 cells treated with or without M-CSF and RANKL for 24 h. c An analysis of the NBCn1 intensity. The bars represent the mean ± s.e.m. (n = 3). d, f Immunofluorescence staining for NBCn1 (red) and DAPI (blue) in Raw264.7 cells (d) and BMMs (f) treated with or without M-CSF and RANKL for 24 h. Scale bar, 10 μm. e, g An analysis of the normalized intensity (total intensity/measuring area) of NBCn1 in Raw264.7 cells (e) and BMM (g) cells. The bars represent the mean ± s.e.m. (n = 5–9). h NBC activity was assessed by measuring the changes in the pHi values of Raw264.7 cells stimulated with or without M-CSF and RANKL for 24 h. i An analysis of NBC activity in Raw264.7 cells (R). The bars represent the mean ± s.e.m. (n = 17). j NBC activity was assessed by measuring changes in the pHi values of BMMs stimulated with or without M-CSF and RANKL for 24 h. k An analysis of NBC activity in BMMs under the indicated conditions. The bars represent the mean ± s.e.m. (n = 3).

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