Fig. 3: ACOT12 deficiency exacerbates lipid accumulation and kidney fibrosis.
From: ACOT12, a novel factor in the pathogenesis of kidney fibrosis, modulates ACBD5
a The KW to BW ratio in UUO-induced Acot12+/+ and Acot12−/− mice (n = 12–19 per group). b Masson’s trichrome and immunohistochemical staining of collagen I and PLIN2 in Acot12+/+ UUO kidneys and UUO Acot12−/− UUO kidneys (n = 3‒6 per group). The positive area was determined and the data are presented as a bar graph. Scale bar for images of Masson’s trichrome staining, 200 μm. Scale bar for images of PLIN2 staining, 100 μm. c The expression levels of inflammatory (Mcp1 and Tgfβ) and fibrotic (Col1a1 and αSma) genes in Acot12+/+ UUO kidneys and Acot12−/− UUO kidneys were analyzed by RT‒PCR and are presented as a heat map (n = 3–6 per group). WTC, wild-type control; WTU, wild-type UUO; ATC, Acot12-/- control; ATU, Acot12-/- UUO. d The expression levels of lipid synthesis genes (Acly, Acaca, Fasn and Scd1) in Acot12+/+ and Acot12−/− UUO kidneys were analyzed via RT‒PCR and are represented as heat maps (n = 3‒6 per group). *P < 0.05, **P < 0.01 and ***P < 0.001 per CL. #P < 0.05, ##P < 0.01 and ###P < 0.001 per Acot12+/+ UUO.
