Fig. 5: Trapping vaspin with vas nanobodies attenuates OA pathogenesis.
From: Blockade of the vaspin–AP-1 axis inhibits arthritis development
a, DMM-operated mice were euthanized at the indicated time points after surgery (n = 5). Safranin O staining images of cartilage sections, OARSI grading and immunostaining image density analysis. b–d, Sham- or DMM-operated WT mice were IA injected with PBS (vehicle) or vas nanobody (25 μg in a total volume of 10 μl) and euthanized at 10 weeks postsurgery (n = 5): schematic of the experimental procedure for vehicle or vas nanobody knee-joint injection, which was initiated at 4 weeks after DMM surgery (b); representative safranin O staining images of joint sections (left) and scoring of OARSI grade (right) (c); representative color densitometry 3D micro-CT images of the medial subchondral bone plate thickness (left; n = 5) and a stacked-bar plot (right) using color to show the trabecular bone thickness distribution in the indicated samples (d). The values are presented as the mean ± s.e.m. and were assessed using a one-way ANOVA with Tukey’s multiple-comparisons test (a), the Mann‒Whitney U test (c) or two-sided chi-square test (d). *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant. Scale bar, 100 μm.
