Fig. 2: LPS stimulation increases fibroblast migrasome release in vitro and in vivo. | Experimental & Molecular Medicine

Fig. 2: LPS stimulation increases fibroblast migrasome release in vitro and in vivo.

From: PGC-1α mediates migrasome secretion accelerating macrophage–myofibroblast transition and contributing to sepsis-associated pulmonary fibrosis

Fig. 2: LPS stimulation increases fibroblast migrasome release in vitro and in vivo.The alternative text for this image may have been generated using AI.

a A typical confocal image of fibroblasts with WGA staining. Enlarged migrasomes are shown in the right panels. The white arrows show migrasomes. Scale bar, 5 µm. b Statistics of migrasome number per cell in migrasome-containing fibroblasts (****P < 0.0001, unpaired t-test, n = 30). c, d The protein expression of migrasome-specific markers integrin α5, NDST1 and TSPAN4 in fibroblasts was determined using western blot (c). The bar plots (d) show the relative expression (*P < 0.05, unpaired t-test, n = 3). e Purified migrasomes derived from fibroblasts were determined using TEM analysis. f Purified migrasomes derived from fibroblasts were determined using western blot. g, h Microscopic inspection (g) of L929 fibroblasts with or without LPS stimulation after scratching (0 h) and after 48 h of wound healing. The bar plots (h) show the relative fibroblast migration area percentage (*P < 0.05, ****P < 0.0001, unpaired t-test, n = 6). i, j The protein expression of migrasome-specific markers integrin α5, NDST1 and TSPAN4 in mouse lung was determined using western blot (i). The bar plots (j) show the relative expression (*P < 0.05, **P < 0.01, unpaired t-test, n = 3). k TEM images of the migrasome observed in normal and fibrotic mouse lung tissues. Scale bar, 2 µm. Enlarged migrasomes are shown in the right panels. Scale bar, 500 nm.

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