Table 1 Key resource details.
Source | Catalog | Reagent or resource |
|---|---|---|
Antibody | ||
Abcam | ab150115 | Goat anti-mouse IgG H&L (Alexa Fluor 647) |
Abcam | ab150160 | Goat anti-rat IgG H&L (Alexa Fluor 594) |
Abcam | ab150105 | Donkey anti-mouse IgG H&L (Alexa Fluor 488) |
Abcam | ab150114 | Goat anti-mouse IgG H&L (Alexa Fluor 555) |
Abcam | ab27156 | Anti-ds DNA antibody (35I9 DNA) – BSA and azide free |
Abcam | ab270993 | Recombinant anti-collagen I antibody (EPR24331-53) |
Abcam | ab252432 | Recombinant anti-mtTFA antibody (EPR23548-120) |
Abcam | ab201693 | Recombinant anti-PIGK antibody (EPR17843) |
Abcam | ab191838 | Anti-PGC1-α antibody |
Abcam | ab5694 | Anti-α smooth muscle actin antibody |
Abcam | ab6640 | Anti-F4/80 antibody (CI:A3-1) |
Abcam | ab7817 | Anti-α smooth muscle actin antibody (1A4) |
Abcam | ab119857 | Anti-CD86 antibody (GL-1) |
Abcam | ab64693 | Anti-mannose receptor antibody |
ABClone | AC026 | β-Actin rabbit mAb |
ABClone | A10253 | TSPAN4/NAG-2 rabbit pAb |
ABClone | A19069 | Integrin alpha 5 (ITGA5/CD49e) rabbit mAb |
BD Pharmingen | 564406 | Fixable viability stain 510 |
BD Pharmingen | 570293 | CD11b RB705 M1/70 |
Biolegend | 103115 | APC/cyanine7 anti-mouse CD45 antibody (30-F11) |
Biolegend | 123115 | APC anti-mouse F4/80 antibody (BM8) |
Biolegend | 101205 | FITC anti-mouse/human CD11b antibody |
Biolegend | 141705 | PE anti-mouse CD206 (MMR) antibody (C068C2) |
Biolegend | 105013 | PE/cyanine7 anti-mouse CD86 antibody(GL-1) |
Biolegend | 137013 | PE anti-mouse CD68 antibody |
Novus | NBP2-34522AF405 | α-SMA antibody (1A4/asm-1) (Alexa Fluor 405) |
SouthernBiotech | 0100-20 | DAPI Fluoromount-G |
SouthernBiotech | 0100-01 | Fluoromount-G |
Proteintech | 66240-1-Ig | β-Tubulin monoclonal antibody |
Proteintech | 26203-1-AP | NDST1 polyclonal antibody |
Beyotime | A0208 | Anti-rabbit IgG, HRP-linked antibody |
Beyotime | A0216 | Anti-mouse IgG, HRP-linked antibody |
Chemicals, peptides and recombinant proteins | ||
MCE | HY-15687A | SAR407899 |
MCE | HY-17538 | ZLN005 |
Sigma | F2006 | Fibronectin human plasma |
Sigma | L2630 | LPS from Escherichia coli O111:B4 |
Sigma | L4516 | LPS from Escherichia coli O127:B8 |
Critical commercial assays | ||
Abcam | ab65321 | mtDNA isolation kit |
invitrogen | M7512 | MitoTracker Red CMXRos |
invitrogen | L3000015 | Lipofectamine 3000 |
invitrogen | W7024 | WGA sampler kit |
Invitrogen | W32466 | WGA |
Miltenyi | 130-095-927 | Lung dissociation kit, mouse |
Sigma | LYSISO1 | Lysosome isolation kit |
Qiagen | 69504 | DNeasy Blood & Tissue Kit (50) |
BD Pharmingen | 554714 | Fixation/permeablization kit |
Pierce | 23227 | BCA protein assay kits |
BD Pharmingen | 555899 | Lysing buffer |
Yeasen | 40203ES76 | CCK-8 |
Vazyme | Q411-02/03 | ChamQ SYBR Color qPCR Master Mix |
Experimental models: cell lines | ||
Raw264.7 | ||
L929 | ||
TSPAN4-mCherry-L929 | ||
TSPAN4-mScarlet-PGC-1α-ZsGreen L929 | ||
Primers | ||
Sangon Biotech | Mouse D-loop | Forward: AATCTACCATCCTCCGTGAAACC |
Reverse: TCAGTTTAGCTACCCCCAAGTTTAA | ||
Mouse Cox1 | Forward: GCCCCAGATATAGCATTCCC | |
Reverse: GTTCATCCTGTTCCTGCTCC | ||
Mouse ND1 | Forward:CTAGCAGAAACAAACCGGGC | |
Reverse:CGGCTGCGTATTCTACGTT | ||
Mouse 18S | Forward: TAGAGGGACAAGTGGCGTTC | |
Reverse: CGCTGAGCCAGTCAGTGT | ||
Mouse B2m | Forward: ATGGGAAGCCGAACATACTG | |
Reverse: CAGTCTCAGTGGG GGTGAAT | ||
Other | ||
Labselect | 14122 | Transwell-Clear inserts, membrane pore size 3.0 μm, polyester (PET) membrane |
Labselect | 14112 | Transwell-Clear inserts, membrane pore size 0.4 μm, polyester (PET) membrane |
Gibco | C11875500BT | RPMI 1640 |
Gibco | 11995065 | DMEM |
Gibco | 15140122 | Penicillin–streptomycin |
Gibco | 12604013 | TrypLE Express |
Software and algorithms | ||
GraphPad Software | Prism 10 | N/A |
ImageJ | ImageJ | N/A |
FlowJo | FlowJo 10 | N/A |
