Fig. 5: LpEV treatment reversed the altered expression of genes for cell proliferation and neurogenesis in the dHP of Drd2-KO mice. | Experimental & Molecular Medicine

Fig. 5: LpEV treatment reversed the altered expression of genes for cell proliferation and neurogenesis in the dHP of Drd2-KO mice.

From: Lactobacillus paracasei-derived extracellular vesicles reverse molecular and behavioral deficits in mouse models of autism spectrum disorder

Fig. 5

a–e, Volcano plots of dHP RNA sequencing datasets (a). RRHO map depicting possible gene expression patterns (b). A RRHO map with gene expression in the dHP of Drd2-KO mice (WT versus KO) and gene expression changed by LpEV in Drd2-KO mice (KO versus KO + LpEV) (c). The top 60% and 40% of ranked genes were selected from quadrants B and C, respectively, for further analysis. d,e, Serial k-means clustering with GO enrichment analysis revealed six and seven functional clusters (k = 6 and 7) with distinct BPs in quadrants B (1595 genes) (d) and C (1885 genes) (e), respectively. f–i, Two functional clusters of genes in quadrant B. Cluster 1 (220 genes) contained genes involved in ‘cell proliferation’ (f). Cluster 2 (279 genes) contained genes regulating ‘neurogenesis’ (h). The interaction networks of genes within the selected BPs are in red (g and i). Clusters 3, 4, 5 and 6 in quadrant B are provided in Supplementary Fig. 6. j–m, Two functional clusters of genes in quadrant C. Cluster 1 (201 genes) harbored genes involved in ‘histone modification and chromatin organization’ (j). Cluster 2 (202 genes) contained genes regulating ‘programmed cell death’ (l). The interaction networks of genes within the selected BPs (blue; k and m). Clusters 3, 4 and 5 in quadrant C are provided in Supplementary Fig. 7.

Back to article page