Fig. 2: PLD6 enhances the oncogenic properties of CRC cells. | Experimental & Molecular Medicine

Fig. 2: PLD6 enhances the oncogenic properties of CRC cells.

From: Phospholipase D6 activates Wnt/β-catenin signaling through mitochondrial metabolic reprogramming to promote tumorigenesis in colorectal cancer

Fig. 2

a The cell viability was assessed using the WST-1 assay in PLD6-KO Caco-2 cells and PLD6-overexpressing HCT116 cells. b The colony-forming assays were performed with Caco-2 and HCT116 cells after manipulating PLD6 expression. c The cell proliferation was analyzed using a two-parameter cell cycle assay, incorporating flow cytometry to evaluate DNA content and BrdU incorporation. d, e The cell migration was evaluated using wound healing and transwell migration assays. For the transwell assay, 1 × 104 Caco-2 cells and 5 × 104 HCT116 cells were seeded into the upper chamber of the transwell. f The invasive potential was assessed using transwell chambers coated with Matrigel, with the same number of cells seeded as in the migration assay. g The changes in EMT markers were detected using western blot analysis of Caco-2 and HCT116 cells. The results represent at least three independent experiments. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001.

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