Fig. 3: PLD6 activity is required for regulating mitochondrial dynamics and membrane potential. | Experimental & Molecular Medicine

Fig. 3: PLD6 activity is required for regulating mitochondrial dynamics and membrane potential.

From: Phospholipase D6 activates Wnt/β-catenin signaling through mitochondrial metabolic reprogramming to promote tumorigenesis in colorectal cancer

Fig. 3

a The representative confocal microscopy images depict the effect of PLD6 KO on mitochondrial morphology in Caco-2 cells. The mitochondrial length and fragmentation were quantified using ImageJ. b The mitochondrial morphology in the indicated HCT116 cells was visualized, with mitochondrial length and fragmentation quantified using ImageJ. c The mitochondrial biomass in vector control and PLD6-altered cells was measured using MitoTracker Red and analyzed via flow cytometry. d The MMP in the indicated CRC cells was assessed using JC-1 dye. e The mitochondrial calcium levels in the indicated cells were measured using Rhod-2AM dye. The representative images were obtained from at least three fields. The results represent at least three independent experiments. Scale bar, 100 μm. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001.

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