Fig. 4: PLD6 plays an oncogenic role in CRC cells by enhancing mitochondrial metabolism and OXPHOS. | Experimental & Molecular Medicine

Fig. 4: PLD6 plays an oncogenic role in CRC cells by enhancing mitochondrial metabolism and OXPHOS.

From: Phospholipase D6 activates Wnt/β-catenin signaling through mitochondrial metabolic reprogramming to promote tumorigenesis in colorectal cancer

Fig. 4

a The expression levels of TCA cycle genes were measured in the indicated cells using qRT–PCR. b The TCA cycle metabolites were quantified in the indicated CRC cells. c The expression levels of the electron transport chain (ETC) complex genes were analyzed using qRT–PCR. d The oxygen consumption rate (OCR) was assessed using the Seahorse Extracellular Flux Analyzer. e The ATP production was measured using the Promega GloMax assay. f The energy phenotype profile, represented as a scatter plot, combines the OCR and extracellular acidification rate (ECAR) analysis. g The mitochondrial ROS levels were visualized using confocal microscopy and quantified. The representative images were obtained from at least three fields. The results represent at least three independent experiments. Scale bar, 100 μm. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001.

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