Table 1 LncRNAs regulating energy metabolism by binding with metabolic enzymes.
From: Long noncoding RNAs regulating enzymatic reactions in cancer
Related metabolism | LncRNAs | Proteins or enzymes | Methods to study binding with lncRNAs | Molecular mechanism | Cancer phenotypes | Cancer types | Reference |
|---|---|---|---|---|---|---|---|
Glycolysis | lncRNA-6195 | ENO1 | RNA-affinity-pull-down–MS, RIP | Inhibits ENO1 enzymatic activity | Inhibits cell proliferation in vitro and HCC growth in vivo | Hepatocellular cancer | |
NEAT1_1 | PGK1, PGAM1, and ENO1 | In vitro RNA pull-down with recombinant PGK1, PGAM1 and ENO1 proteins, fluorescence polarization assay, cross-linking IP-qPCR | Accelerates glycolysis by substrate channeling of PGK1, PGAM1, and ENO1 | Promotes cell proliferation and metastasis | Breast cancer | ||
gLINC | PGK1, PGAM1, ENO1, PKM2 and LDHA | RNA-affinity pull-down, sucrose density gradient centrifugation, IF, co-IP, fluorescence in situ hybridization (FISH), RIP | Enhances glycolytic flux by channeling of PGK1, PGAM1, ENO1, PKM2, and LDHA | Promotes cell proliferation in vitro and tumor growth in vivo | Hepatocellular cancer, CRC and cervical cancer | ||
HULC | LDHA and PKM2 | Surface plasmon resonance, TOBAP-MS | Enhances phosphorylation of both LDHA, and PKM2, then increases glycolysis | Promotes cell proliferation in vitro | Hepatocellular cancer | ||
WFDC21P | PFKP and PKM2 | RIP, IP using MS2-tagged RNA aptamer | Disrupts the PFKP tetramer formation and inhibits of PKM2 nuclear translocation | Inhibits cell proliferation, metastasis in vitro and tumor growth in vivo | Hepatocellular cancer | ||
TCA cycle | TINCR | ACLY | RNA-affinity-pull-down–MS, RNA-EMSA | Protects from ubiquitination and maintains concentration of acetyl-CoA | Promotes cell proliferation, migration and invasion in vitro and tumor growth in vivo | Nasopharyngeal cancer | |
IDH1-AS1 | IDH1 | RNA-affinity pull-down, RIP | Enhances the enzymatic activity by dimerization of IDH1 | Inhibits cell proliferation in vitro and tumor growth in vivo | Cervical cancer | ||
GAS5 | MDH2 and CS | RNA-affinity-pull-down–MS, co-IP | Disrupts FH, MDH2, and CS interactions | Inhibits cell proliferation in vitro and tumor growth in vivo | Breast cancer | ||
AC020978 | MDH2 | RNA-affinity-pull-down–MS, RIP | Protects from ubiquitination and activates the AKT signaling pathway by binding with MDH2 | Promotes cell migration and invasion in vitro and metastasis in vivo | NSCLC | ||
Arginine | LOC113230 | ASS1 | RNA-affinity-pull-down–MS, RIP, co-IP | Facilitates assembly of LRPPRC–TRAF2 E3 complex and induces ubiquitination on K234 residue | Inhibits cell proliferation and migration in vitro and tumor growth in vivo | CRC |
