Fig. 1: Establishment and characterization of Taxol-resistant TNBC cells.
a A schematic representation of the protocol used for generating Taxol-resistant SUM159PT cells. The cells were exposed to increasing concentrations of Taxol (IC10 or IC50) for 72 h, with subsequent doubling of the drug amount upon confluence. This cycle was repeated until the IC50 values of the cells significantly differed from the initial cell population. Created with BioRender.com. b IC50 values for T1-160 (generated as a resistant cell to the folds of IC10 value of Taxol) and T2-450 (resistant to the folds of IC50). c Colony formation assay in the presence of indicated amounts of Taxol. d Quantification of colony areas in c. e A comparison of growth rates between parental and Taxol-resistant SUM159PT cells. f Immunofluorescence staining for α-tubulin (green) and DAPI (blue) after 4 h of exposure to DMSO or Taxol (parental: 160 nM, T1-160: 160 nM, T2-450: 450 nM). Scale bar: 50 µm. g Quantification of cell area in f. h AnnexinV/Dead cell assay after 24 h of DMSO or Taxol treatment (parental: 160 nM, T1-160: 160 nM, T2-450: 450 nM). i Western blot analysis of the cells shown in h for total PARP, cleaved-PARP (c-PARP), cleaved-caspase3 (c-Caspase 3) and GAPDH as a loading control. j Cell cycle assay after 8 h of DMSO or Taxol treatment (parental: 160 nM, T1-160: 160 nM, T2-450: 450 nM). For statistical analysis, each Taxol group was compared with same cell’s DMSO group. P values determined by two-tailed Student’s t-test in comparison with control group; *P < 0.05, **P < 0.01, ***P < 0.001.
