Fig. 5: Effects of mitochondrial transplantation on mitophagy in BTHS CMs. | Experimental & Molecular Medicine

Fig. 5: Effects of mitochondrial transplantation on mitophagy in BTHS CMs.

From: Allogenic mitochondria transfer improves cardiac function in iPS-cell-differentiated cardiomyocytes of a patient with Barth syndrome

Fig. 5

a Effects of mitochondrial transplantation on mitochondrial morphology in BTHS CMs. Top: normal and BTHS CMs were stained with MitoFlamma (Deep Red) at the indicated times after mitochondria transplantation. Bottom: magnified images of the dotted area are shown. Arrowheads indicate mitochondria. Scale bar, 20 μm. b The mitochondrial perimeter was measured using ImageJ software. Data are presented as mean ± s.d. (n = ~39–91). c Effects of mitochondrial transplantation on mitophagy and autophagy in BTHS CMs. After transplantation of normal mitochondria into BTHS CMs, the expression and the phosphorylation levels of LC3, AMPKa, ULK, p70S6K and GAPDH were determined by western blot analysis 48 h after transplantation. d Effects of mitochondrial transplantation on mitochondrial morphology in BTHS CMs. Mitochondrial morphology of normal CMs, BTHS CMs and mitochondria-transplanted BTHS CMs analyzed by TEM analysis. Mitochondria (M) and autophagosome (A) are indicated. Scale bar, 1 μm. e Quantification of mitochondria fused with autophagosomes in TEM images. Data are presented as mean ± s.d. (n = ~10–12). f, g Cristae junction (f) and width (g) in the TEM images were measured by ImageJ software. Data are presented as mean ± s.d. (n = ~125–231). Statistical significance was calculated using one-way ANOVA with Holm–Šidák’s multiple-comparisons test, and P values are shown in the figures.

Back to article page