Fig. 6: Effects of mitochondrial transplantation on expression of cardiac structure markers. | Experimental & Molecular Medicine

Fig. 6: Effects of mitochondrial transplantation on expression of cardiac structure markers.

From: Allogenic mitochondria transfer improves cardiac function in iPS-cell-differentiated cardiomyocytes of a patient with Barth syndrome

Fig. 6

a Immunostaining of cTnT and MLC2v in normal CMs, BTHS CMs and BTHS CM + MT. Normal CMs, BTHS CMs and BTHS CMs transplanted with MT (48 h after transplantation) were stained with anti-cTnT antibody (green color), anti-MLC2v antibody (red color) and DAPI (blue color), respectively. Scale bar, 20 μm. b Effects of mitochondrial transplantation on expression of cardiac markers. Left: the expression levels of cardiac markers (α-SA, cTnT and cTnI) and GAPDH were determined by western blotting in normal CMs, BTHS CMs and BTHS CM + MT. Right: the protein band intensities were quantified using ImageJ software, and data are presented as mean ± s.d. (n = ~4–5). Statistical significance was calculated using one-way ANOVA with Holm–Šidák’s multiple-comparisons test, and P values are shown in the figures.

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