Fig. 2: Effects of the dose and duration of A62M and A62D-8T on IR phosphorylation and downstream signaling.
From: Structural mechanism of insulin receptor activation by a dimeric aptamer agonist
a–f Comparison of dose-dependent phosphorylation of IR and downstream signaling by A62M or A62D-8T. Rat-1/hIR cells were incubated with varying concentrations of A62M or A62D for 1 h. The relative band intensities, compared with 100 nM insulin, are shown for pIR Y1150 (a) pIR Y1150/Y1151 (b) pIR pY (c) pAKT S473 (d) pAKT T308 (e) and pERK T202/Y204 (f) presented as the mean ± s.d. of three independent replicates. g–k Time-dependent phosphorylation of IR and downstream signaling. Rat-1/hIR cells were stimulated with 50 nM insulin for 10 min or 200 nM A62M-8T for 5 min to 4 h. The kinetics of pIR Y1150 (g) pIR Y1150/Y1151 (h) pAKT T308 (i), pAKT S473 (j) and pERK T202/Y204 (k) are presented as the mean ± s.d. of three independent experiments, normalized to each negative control (NT) to determine the fold change from basal levels.
