Fig. 5: Kctd17 inhibits Zbtb7b-mediated Serpina3k expression. | Experimental & Molecular Medicine

Fig. 5: Kctd17 inhibits Zbtb7b-mediated Serpina3k expression.

From: Hepatocyte KCTD17-mediated SERPINA3 inhibition determines liver fibrosis in metabolic dysfunction-associated steatohepatitis

Fig. 5

a Serpina3k mRNA expression from Hepa1c1c7 cells transfected with HA/Zbtb7b. b Zbtb7b expression from Hepa1c1c7 cells transfected with HA/Kctd17. c Zbtb7b expression in the liver from FPC diet-fed AAV8-TBG or AAV8-TBG-Kctd17 mice (n = 8 per group). d Western blots in Hepa1c1c7 cells transfected with HA/Zbtb7b and Kctd17/Flag with or without MG-132. e, f Co-immunoprecipitation of HA/Zbtb7b, Kctd17/Flag (e) and Myc/Cul3 (f) in Hepa1c1c7 cells with MG-132. g Zbtb7b ubiquitination in 3x Flag/Ub and Kctd17/Flag-transfected Hepa1c1c7 cells. h Immunofluorescent staining in EV or HA/Kctd17-transfected Hepa1c1c7 cells (n = 6 per group; scale bar, 10 μm; magnification, 60×). i Serpina3k mRNA expression from Hepa1c1c7 cells transfected with HA/Zbtb7b and/or Kctd17/Flag as indicated. **P < 0.01, ***P < 0.001 compared with the indicated EV control, #P < 0.05 compared with Kctd17, +P < 0.05 compared with Zbtb7b according to a two-way analysis of variance. All data are shown as the means ± s.e.m.

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