Fig. 3: Hippocampal astrocytes in HFD/STZ-induced mice exhibited an atrophic morphology and decreased synaptic coverage. | Experimental & Molecular Medicine

Fig. 3: Hippocampal astrocytes in HFD/STZ-induced mice exhibited an atrophic morphology and decreased synaptic coverage.

From: Cholesterol-driven pathological astrocytic responses in diabetes-associated cognitive impairment through astrocytic SCAP accumulation and NF-κB–C3 signaling modulation

Fig. 3

a Representative z-projection images of GFAP- and DAPI-labeled hippocampal subregions surveyed in all groups. Scale bar, 20 μm. The insets show higher magnifications (top) and 3D rendering (bottom) of the boxed areas in the respective panels. Scale bar, 10 μm. The brain tissues from five independent experiments were analyzed (n = 5 per group). b, c The quantification of astrocyte volume (b) and surface area (c) in hippocampal subregions. n = 4–5 cells/animal. d–g The quantification of the astrocyte soma volume (d), branch length (e), branch number (f) and branch endpoints (g). h The representative images of SYP coverage with astrocytes in the hippocampus in each group. Scale bar, 10 μm. The insets show the 3D rendering of boxed areas in the respective panels. i, j The quantification of the number (i) and volume (j) of SYP-positive puncta colocalized with GFAP per astrocyte in the hippocampus. The data are presented as the means ± s.e.m. A two-tailed unpaired Student’s t-test was used. n.s., nonsignificant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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