Fig. 7: HOIL-1 promotes HBx stabilization via recruiting deubiquitinating enzyme USP15. | Experimental & Molecular Medicine

Fig. 7: HOIL-1 promotes HBx stabilization via recruiting deubiquitinating enzyme USP15.

From: The LUBAC subunit HOIL-1 promotes the progression of HBV-associated hepatocellular carcinoma independently of linear ubiquitination

Fig. 7

a The western blot of indicated proteins detected in HepG2.2.15 cells. b The CCK-8 assays detected the effect of USP15 knockdown on HepG2.2.15 transduced with LV-HOIL-1 or LV-Ctrl. c The EdU incorporation detected the effect of USP15 knockdown on HepG2.2.15 transduced with LV-HOIL-1 or LV-Ctrl. d The Co-IP for the K48-linked ubiquitination of HBx in the HepG2.2.15 cells after 8 h of MG132 treatment (10 μM). e The Co-IP and blotting of anti-HOIL-1 or anti-HBx or USP15 in HepG2.2.15 cells. f The Co-IP and blotting of anti-HOIL-1 or anti-HBx or USP15 in HepG2.2.15 cells. g The representative images of the IHC staining of HOIL-1, HBx and USP15 in HCC tissues and the correlation between HOIL-1 and HBx levels. h The Kaplan–Meier survival analysis of patients in the HBV-HCC cohort (GSE14520) stratified by USP15 and HOIL-1 expression. The patients were divided into USP15-high and USP15-low subgroups and, within each subgroup, further stratified by HOIL-1 expression levels. The Mann–Whitney U test was used in b and c, Spearman’s rank correlation test in g and the log-rank Mantel–Cox test in h. Data are shown as mean ± s.d., *P < 0.05, **P < 0.01.

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