Fig. 2: HnRNPU knockdown inhibits cell proliferation and induces cell cycle arrest. | Experimental & Molecular Medicine

Fig. 2: HnRNPU knockdown inhibits cell proliferation and induces cell cycle arrest.

From: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc−

Fig. 2: HnRNPU knockdown inhibits cell proliferation and induces cell cycle arrest.The alternative text for this image may have been generated using AI.

a Western blot analysis were performed to detect HnRNPU expression in SW620 and SW480 cells with sh-HnRNPU transfection. GAPDH was included as an internal control. b Cell viability was determined using CCK-8 in SW620 and SW480 cells with HnRNPU knockdown. c, d The effects of HnRNPU knockdown on cell proliferation were determined using the colony formation assay. e The effects of HnRNPU knockdown on DNA replication were determined using the EdU staining. Scale bar, 100 μm. f The cell cycle distributions of SW620 and SW480 cells with HnRNPU knockdown were detected using flow cytometry. g Cell-cycle-related proteins were detected using western blot analysis in SW620 and SW480 cells with HnRNPU knockdown. P value significant codes: *P < 0.05; **P < 0.01.

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