Fig. 3: HnRNPU knockdown induces ferroptosis in COAD by inhibiting system xc−/GSH/GPX4 axis. | Experimental & Molecular Medicine

Fig. 3: HnRNPU knockdown induces ferroptosis in COAD by inhibiting system xc−/GSH/GPX4 axis.

From: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc−

Fig. 3: HnRNPU knockdown induces ferroptosis in COAD by inhibiting system xc−/GSH/GPX4 axis.The alternative text for this image may have been generated using AI.

a The differentially expressed genes were identified using RNA sequencing in SW620 cells with HnRNPU knockdown. Significantly upregulated and downregulated proteins are highlighted in red and blue, respectively. The curve is derived with a false discovery rate of 0.05. b Cell viability was determined using CCK-8 in SW620 and SW480 cells with HnRNPU knockdown in the absence or presence of liproxstatin-1 (1 μM), necrosulfonamide (1 μM), Z-VAD-FMK (10 μM) or 3-methyladenine (200 μM) for 24 h. c The expression of GPX4, SLC3A2 and SLC7A11 was detected in HnRNPU-knockdown cells by using western blot analysis. HSP90 was included as an internal control. d, e The level of GSH and the ratio of GSH/GSSG were determined in HnRNPU-knockdown cells. f Cystine uptake capacity was determined in HnRNPU-knockdown cells. P value significant codes: *P < 0.05; **P < 0.01.

Back to article page