Fig. 4: HnRNPU knockdown enhances the sensitivity of COAD cells to ferroptosis. | Experimental & Molecular Medicine

Fig. 4: HnRNPU knockdown enhances the sensitivity of COAD cells to ferroptosis.

From: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc−

Fig. 4: HnRNPU knockdown enhances the sensitivity of COAD cells to ferroptosis.The alternative text for this image may have been generated using AI.

a Cell viability was determined using CCK-8 in SW620 and SW480 cells expressing sh-con or sh-HnRNPU treated with different concentrations of RSL3 for 24 h. b MDA assay kit was used to detect the level of MDA treated with RSL3 (SW620:RSL3 5 μM; SW480:RSL3 7.5 μM). c An electron microscopy technique was applied to determine the changes occurring in the cellular mitochondria to assess ferroptosis. Scale bar, 0.5 μm. d The cell viability was determined using CCK-8 in SW620 and SW480 cells expressing sh-con or sh-HnRNPU treated with RSL3 in the absence or presence of liproxstatin-1 (1 μM), necrosulfonamide (1 μM), Z-VAD-FMK (10 μM) or 3-methyladenine (200 μM) for 24 h (SW620:RSL3 5 μM; SW480:RSL3 7.5 μM). e Cell viability was determined using CCK-8 in SW620 and SW480 cells expressing sh-con or sh-HnRNPU cultured under cystine restriction for 24 h alone or in combination with liproxstatin-1 as indicated is shown. P value significant codes: *P < 0.05; **P < 0.01.

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