Fig. 5: HnRNPU promotes SLC7A11 and SLC3A2 mRNA stability in COAD cells. | Experimental & Molecular Medicine

Fig. 5: HnRNPU promotes SLC7A11 and SLC3A2 mRNA stability in COAD cells.

From: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc−

Fig. 5: HnRNPU promotes SLC7A11 and SLC3A2 mRNA stability in COAD cells.The alternative text for this image may have been generated using AI.

a, b RT–qPCR was performed to detect mRNA expression of SLC7A11 and SLC3A2 in SW620 (a) and SW480 (b) cells with HnRNPU-knockdown. c, d RT–qPCR was performed to evaluate the mRNA stability of SLC7A11 and SLC3A2 after adding actinomycin D in SW620 (c) and SW480 (d) cells with HnRNPU-knockdown. e RIP assay was used to analyzed the interaction between HnRNPU and SLC7A11, along with SLC3A2 in SW620 cells. f RIP assay was used to analyze the interaction between HnRNPU and SLC7A11, along with SLC3A2 in HnRNPU-knockdown cells. g Dual-luciferase reporter assays were used with SLC3A2-5′ UTR/CDS/3′ UTR/ and SLC7A11-5′ UTR/CDS/3′ UTR#1/3′ UTR#2. P value significant codes: *P < 0.05; **P < 0.01, ***P < 0.001.

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