Fig. 3: Chemically prepared neo-glycoRNA has different properties from N-glycoconjugates.
From: RNA-associated glycoconjugates highlight potential ambiguities in glycoRNA analysis
a Schematics for preparing DBCO-functionalized RNA for click strain-promoted click reaction. b Denaturing agarose gel (1%) electrophoresis and in-gel fluorescence of Cy5-clicked, DBCO-functionalized RNA. The EtBr-stained gel was scanned after the fluorescence scan of the same gel. c Schematics for preparing N-glycan conjugated neo-glycoRNA. d Denaturing agarose gel (1%) electrophoresis and in-gel fluorescence of neo-glycoRNA and RNase-treated samples. The pink line next to the AF555 fluorescent gel indicates the position of the EtBr signal of the same gel. The AF555 signal has a highly similar mobility to EtBr signals. AF555, AlexaFluor-555; CMP, cytidine monophosphate; DBCO, dibenzocyclooctyne; LED, light-emitting diode; ST6Gal1, β-galactoside α-2,6-sialyltransferase 1.
