Fig. 4: PTBP1 KD rescues hippocampal neuron loss by inhibiting apoptosis. | Experimental & Molecular Medicine

Fig. 4: PTBP1 KD rescues hippocampal neuron loss by inhibiting apoptosis.

From: The therapeutic potential and mechanisms of targeting the PTBP1/Nogo-A/NgR axis in PTSD induced by single prolonged stress in mice

Fig. 4: PTBP1 KD rescues hippocampal neuron loss by inhibiting apoptosis.The alternative text for this image may have been generated using AI.

a TUNEL staining of the hippocampus in mouse brain. Scale bar, 100 µm. b Quantitative analysis of TUNEL-positive cells in the DG region. c Immunofluorescence staining for NeuN (red). Scale bar, 100 µm. d Statistical analysis of mean fluorescence intensity of NeuN. e Immunofluorescence staining for Bcl-2 (red). Scale bar, 50 µm. f Statistical analysis of mean fluorescence intensity of Bcl-2. g Western blot analysis of NeuN and apoptosis-related proteins, including Bcl-2, Bax, Caspase-3 and Cleaved Caspase-3. h–l Quantitative analysis of western blot results for NeuN (h), Bcl-2 (i), Bax (j), Caspase-3 (k) and Cleaved Caspase-3 (l). m Heatmap representation of western blot experimental results. Data are expressed as mean ± s.e.m. (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.

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