Fig. 6: Regulation of axonal regeneration by PTBP1 KD via the Nogo-A/NgR/RhoA/ROCK2 axis in normal and PTSD mice. | Experimental & Molecular Medicine

Fig. 6: Regulation of axonal regeneration by PTBP1 KD via the Nogo-A/NgR/RhoA/ROCK2 axis in normal and PTSD mice.

From: The therapeutic potential and mechanisms of targeting the PTBP1/Nogo-A/NgR axis in PTSD induced by single prolonged stress in mice

Fig. 6: Regulation of axonal regeneration by PTBP1 KD via the Nogo-A/NgR/RhoA/ROCK2 axis in normal and PTSD mice.

a, b Immunofluorescence staining of NF200 (green) (a) and GAP-43 (red) (b) in the DG region of the hippocampus. Scale bar, 100 µm. c, d Statistical analysis of the average fluorescence intensity of NF200 (c) and GAP-43 (d). e Western blot analysis of NF200, GAP-43, Nogo-A, NgR, RhoA and ROCK2. f–k Quantitative analysis of western blot results for NF200 (f), GAP-43 (g), Nogo-A (h), NgR (i), RhoA (j) and ROCK2 (k). l The heatmap representation of western blot results. m Western blot analysis of PTBP1 KD-induced changes in the Nogo-A/NgR/RhoA/ROCK2 pathway and NF200/GAP-43 in normal mice. n Quantitative analysis of western blot results for the Nogo-A/NgR/RhoA/ROCK2 pathway and NF200/GAP-43 in normal mice. Data are expressed as mean ± s.e.m. (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001. ****P < 0.0001; ns, not significant.

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