Fig. 1: Muscle stem/progenitor cells on the concave side of patients with AIS are exposed to high ROS levels and exhibit suppressed METTL3 expression.

a,b Bubble chart of GO analysis of upregulated (a) and downregulated (b) genes in paraspinal muscle tissues collected from the concave side compared with those from the convex side in patients with AIS (n = 3). c Relative ROS intensity in bilateral paraspinal muscles derived from patients with CS (n = 10) and AIS (n = 9). ***P < 0.001; ns indicates no significant changes. d,e Bubble chart of GO analysis of upregulated (d) and downregulated (e) genes in muscle stem/progenitor cells collected from concave paraspinal muscles compared those from convex paraspinal muscles in patients with AIS. f Heat map representation of downregulated genes in concave paraspinal muscle stem/progenitor cells in GO term RNA methyltransferase activity. g Relative mRNA expression of METTL3 in bilateral muscle stem/progenitor cells collected from the patients with CS (n = 10) and AIS (n = 9). **P < 0.01; ns indicates no significant changes. h The protein level of METTL3 of bilateral muscle stem/progenitor cells collected from patients with AIS. GAPDH served as the internal reference. i The relative global m⁶A levels in bilateral muscle stem/progenitor cells collected from the patients with CS (n = 10) and AIS (n = 9). **P < 0.01; ns indicates no significant changes.